MICROBIAL GROWTH OF LACTOBACILLUS DELBRUECKII SSP. BULGARICUS B1 IN A COMPLEX NUTRIENT MEDIUM (MRS-BROTH)

Georgi Kostov; Rositsa Denkova-Kostova; Vesela Shopska; Bogdan Goranov; Zapryana Denkova

doi:10.26034/lu.akwi.2022.3479

Authors

Georgi Kostov Department of Wine and Beer, University of Food Technologies, 4002, 26 Maritza Blvd., Plovdiv, Bulgaria
Rositsa Denkova-Kostova Department of Biochemistry and Molecular Biology, University of Food Technologies, 4002, 26 Maritza Blvd., Plovdiv, Bulgaria
Vesela Shopska Department of Wine and Beer, University of Food Technologies, 4002, 26 Maritza Blvd., Plovdiv, Bulgaria
Bogdan Goranov Department of Microbiology University of Food Technologies, 4002, 26 Maritza Blvd., Plovdiv, Bulgaria
Zapryana Denkova Department of Microbiology University of Food Technologies, 4002, 26 Maritza Blvd., Plovdiv, Bulgaria

DOI:

https://doi.org/10.26034/lu.akwi.2022.3479

Keywords:

Probiotics, growth kinetics, modeling, optimization, complex nutrient medium, MRS-broth

Abstract

The microbial growth of the probiotic strain Lactobacillus delbrueckii ssp. bulgaricus B1, cultivated in a complex nutrient medium (MRS-broth), was studied in the present work. The complex nutrient medium provides not only the carbon source necessary for the growth of biomass, but also all the additional sources of nitrogen, phosphorus and other components that the biomass needs for its growth. The use of non-structural mathematical dependences determines the optimal conditions (substrate concentration) for the accumulation of biomass or lactic acid, depending on the needs of the specific production.